Wednesday, April 17, 2013

Chicken Leg Dissection



Procedure:
Put on gloves and collect your tools.
Thoroughly rinse and dry one chicken leg. Place it in the dissecting tray.


















Lab Assistant.













Examine the outside skin tissue. This is the epidermis and the dimples are where feathers emerged. Using scissors and forceps.

Cut the skin and peel it away from the muscle.

Notice the clear connective tissue that holds the skin to the muscles. As you peel off the skin, you may need to cut away some of this connective tissue. Work slowly and carefully with scissors and forceps until all skin is removed.













Describe how the connective tissue looks and feels. What type of connective tissue is this? The connective tissue looks shiny and flimsy and is stretchy. It feels very smooth. This is epithelial tissue.

Observe, with your naked eye bundles of muscle tissue surrounding the bones. Separate the bundles of muscles by separating them out with your fingers. Begin by inserting your thumb into the muscle of the lower leg. You will need to push forcefully through the shiny lining (called fascia) over the muscle, but it will give way at the natural separations between the muscle bundles. Continue separating the muscle into bundles by forcing your thumb and fingers through the muscle until you are able to distinguish several separate bundles.













Describe the arrangement of the muscle bundles. The muscle bundles are thick and close together. 

The strong, shiny, white cords, called tendons, hold the muscle to the bones. Some of these tendons will pull away from the bone as you separate the muscle bundles. Use a probe, if needed, to find the tendons of the chicken leg. Using the dissection scissors, cut across the tendons at Line A (Figure 1). Observe the numerous tendons and pull the freed muscles down and away from the bone, as if you were peeling a banana. Careful you don't cut any ligaments that attach bone to bone. Look closely at the ligaments.













Examine the two bones in the lower leg. The large bone (Bone A) is the tibia. The small, toothpick-like bone (Bone B) is the fibula.




 What sort of connective tissue are tendons composed of? Tendons are composed of fibrous connective tissue.

Remove a single muscle by cutting the tendons and peeling the muscle away from the bone.

What sort of muscle tissue is represented? How do you know? This is skeletal muscle because it connects muscle to bone.

Nerves are generally thin, threadlike white strands found between the muscle and the
nearest bone. Look for the nerve in your specimen. Did you find them? I saw some very thin white that might have been nerves but it was kind of hard to tell. 

What is the physical difference in the tendon of the insertion when compared to the origin? The insertion was longer and thinner then the origin.

Remove all remaining muscle to expose the bones of the chicken leg.

What is the soft material inside a bone? The soft material inside a bone is marrow. 

Name three specific types of cells present here. Do not break the bone; it is sharp! Osteocytes, osteoblasts, and osteoclasts.

Name three functions of bone. Three functions of bone are movement, support, and protection.

Cut onto the hinge joint by cutting into the top of the covering of the joint from the femur side. It will become apparent that you must remove the knee cap area to expose the menisci and ligaments within. Pull up on the knee cap area and cut through it with the scissors. You will have cut through the bursa, a sac that acts as a shock absorber for the knee joint. These are found in every joint.

Pull the covering back and look into the inside of the joint. You will see more white bands of ligaments holding the bones together. Observe the shiny, white layer covering the ends of the bones is cartilage. It helps the bones slide smoothly when the leg bends.













Bend the specimen at Joint B (Figure 1) and rotate the femur in all directions. Remove the muscle that covers Joint B by cutting parallel to the femur, upward toward the backbone. Remove pink muscle tissue until you see a shiny white sheet of ligament that covers the joint. Present is an exterior ligament that holds the femur in the hip socket

What type of connective tissue composes the ligaments? Ligaments are composed of dense connective tissue.
Figure 2 is provided in a separate document.
Label Figure 2 with the names of the joints you observed and the motion they make.

On Figure 2, sketch one muscle origin (the name of the bone indicates the insertion) and one muscle insertion you can see in the leg.


















Hinge joints allow for movement on one plane. 

Ball-and-socket joints permit even wider ranges of movement. The rounded head of the bone fits into a socket, allowing movement in all planes. 

Tuesday, March 26, 2013

Unit 2 Reflections



Unit 2 Reflections

A topic that I felt engaged by in unit two was chromosomal alterations, specifically Down syndrome or Trisomy 21. I have recently been following two family blogs who have young children with Down syndrome. I enjoy reading both of these blogs and wanted to understand more what Down syndrome is and how it affects individuals. I think sometimes as a society in general we are afraid of people that are different from us and the unknown. Through these blogs I have learned these children are just like mine with the exception of having some medical issues that can be quite serious, however they are just like other children playing, enjoying life, and just being children. 

Down syndrome or Trisomy 21 is caused by inheriting an extra copy of the twenty first chromosome.  Some interesting information I learned while doing my research is that Down syndrome is the most common chromosome abnormality in humans. The CDC estimates that yearly one in 691 births results in a baby with Down syndrome, I had no idea how prevalent Down syndrome is. Some characteristics of Down syndrome are developmental delays that can be mild or very profound and distinct physical features such as a flat facial profile, an upward slant to their eyes, small ears and a protruding tongue.  Some risk factors for Down syndrome include advancing maternal age, having had a child with Down syndrome or parents who are carriers of the genetic translocation for Down syndrome. I was aware that advanced maternal age was a risk factor but didn’t know about the other potential causes.  
















Photo credit:  http://kgiegler.wordpress.com/2011/03/06/downs-syndrome-101/ Retrieved March 26, 2013




















Photo credit: schoolworkhelper.net/down-syndrome-causes-symptoms-treatment   Retrieved March 26, 2013

The outlook for individuals with Down syndrome has increased drastically in recent years. This is due in part to better medical treatment and social inclusion. Life expectancy is still reduced compared to the normal population. An individual with Down syndrome could live to age fifty and beyond depending on the severity of their health problems. From the two blogs I have read I understand there are many challenges that come with raising children with Down syndrome but also immeasurable joy. Down syndrome is not something that needs to be feared, it can be managed and the individuals can lead fulfilling lives. 

Sources

Wikipedia http://en.wikipedia.org/wiki/Down_syndrome  Retrieved March 26, 2013 

Down Syndrome, Sietske N Heyn PhD, http://www.medicinenet.com/down_syndrome/article.htm Retrieved March 26, 2013

About Down Syndrome, Kids Health http://kidshealth.org/parent/medical/genetic/down_syndrome.html  Retrieved March 26, 2013

Part 2. Thinking about the course
1.       The assignment for unit 2 that I am most proud of is my unit paper. I really felt engaged by the topic I chose and enjoyed learning more about Down syndrome. I had a personal interest in it which seems to add to the enjoyment of learning about a subject.
2.       I feel like I now have a pretty good understanding of mitosis and meiosis. While studying for my exam it seemed to come together better for me. Some of the phases etc are still confusing but I think I have the basics down.
3.       During this unit I made flashcards to study the terms and spent a good amount of time on the end of the chapter reviews. Those reviews are very thorough and seem to help my understanding and help me to know where to focus my time.
4.       I felt most connected with the course during this unit while learning about the inheritance of traits and some of the disorders. I liked learning about how we as individuals get our phenotypes; it’s not something I have put much thought into prior to this Biology class. I also like reading about the background of the disorders, you hear about people who have them but I haven’t spent much time understanding them.  While I enjoyed learning about the traits I think I felt disconnected while learning the way the traits come to be, using punnett squares and trying to figure out how and why things cross was confusing for me.
5.       I think just like in Unit 1 that you offer us plenty of different opportunities to learn and review the material. I had some personal things change during this unit with my work schedule etc that provided extra challenges for me and I feel like I didn’t spend as much time on the material as I could have.

Monday, March 4, 2013




Reproductive Cloning

Reproductive cloning is making an exact copy of an organism that already exists or has existed in the past. In 1997 researchers cloned an adult sheep, Dolly. Dolly was genetically identical to the adult she was cloned from. Reproductive cloning in animals is accomplished by a process called somatic cell nuclear transfer. In somatic cell nuclear transfer genetic material from the nucleus of a donor adult cell is transferred to an egg whose nucleus with its genetic material has been removed. The resulting egg containing the donors DNA must be treated with chemicals or electric current to stimulate cell division. Once the cloned egg reaches a suitable stage it is transferred to the uterus of a female host where it will continue to develop until birth. 

So why clone? Our Biology book openly states that besides the novelty of actually cloning a human for the first time, this concept is likely to be of little practical value in the near future. (Johnson, 405) I think it is important to note that while human cloning seems to carry the most controversy, scientists have been cloning animals for years already.  When doing my internet research I came across some other reasons people give for justifying cloning. Some of the reasons are medical purposes, reviving endangered or extinct animals species, helping infertile couples have children, and cloning stem cells for research. 

When we hear about cloning, we often only hear about the successful cases, Dolly being a bright example of this. Two big issues with cloning are the high failure rate of the procedure and problems during later development. In the case of the famous Dolly she died at age 6 from progressive lung disease and had also contracted arthritis prematurely. The type of lung disease Dolly had is more common in older sheep. Some clones have developed other issues such as kidney and brain malformations and impaired immune systems. 

So the debate ensues, to clone or not to clone? Scientists and researchers and others support cloning because of the wide variety of issues that cloning could help.  Some are against cloning because of the really large amount of unknown variables and the ethical issues. Cloning seems like it could have some very productive places in society but I think we need to make sure we know exactly what we are doing and the implications that it carries. It feels like in the process of learning how to clone we lose sight of some of the moral and ethical issues that arise when not thinking through all the involved aspects of cloning. I do support progress in treating illness and disease. Having suffered from infertility I can even see how cloning may be appealing to some but I cannot say I would have considered this route while we were trying to add to our family. I do not support cloning simply for the novelty of it.

 Sources

Therapeutic Cloning vs. Reproductive Cloning
Accessed March 3, 2013.

Why Clone.
The University of Utah. 
Accessed March 4, 2013.

Cloning Risks.
Accessed March 4, 2013

Dolly the Sheep dies young.
Will Knight.
Accessed March 4, 2013

Human Biology. Sixth Edition. Michael D. Johnson

Wednesday, February 20, 2013



Stem Cell Research

Parkinson’s disease, leukemia, Alzheimer’s disease, and spinal cord injuries are all diseases or injuries that may benefit from stem cell research, as well as many others. Stem cells are the body’s raw materials, cells from which all other cells with specialized functions are generated. No other cell in the human body has the natural ability to generate new cell types.  Stem cells have the capacity to become whatever type of specialized cell a patient needs. Stem cells come from embryos, adult stem cells, adult stem cells that are altered to have properties of embryonic stem cells, and have also been found in amniotic fluid.  Since it seems stem cell research has great potential to cure debilitating diseases why would anyone object to this research? Controversy comes in specifically with the use of human embryonic stem cells. I chose this topic because I do not know much about it and wanted to research it for myself to see how I feel about the issue.


 












In my research I learned that the most desirable stem cells come from early stage embryos.  These embryos are usually obtained from eggs that were fertilized at in vitro clinics but were never implanted because they were not needed or wanted. This is where the ethical issue lies. Adult stem cells are being used but do not offer as much versatility as embryonic stem cells and are not able to be manipulated as much to produce all the cell types. However advances are being made in adult stem cell research and there is promise in that area. The biggest controversy boils down to when an embryo is actually considered a human life. Those who value human life from the point of conception consider the destruction of the embryo to be killing a human life while research advocates feel that the tiny blastocyst does not have human characteristics.  

So what is the solution?  I see both sides; depending on where your beliefs lie it is a sensitive subject. I feel like we should not specifically make the blastocysts for research but if there are unused embryos and they were to be donated for research so that others may live it feels like it could be positive.  I tried to put myself in the position of having a very sick child or family member and how I would feel if they could be cured by stem cells. Our textbook talked about advances being made in adult stem cell research that may make embryonic stem cell research unnecessary some day, that would be an amazing accomplishment and would solve the ethical issue.

Sources
Photo credit.  Retrieval date 2-19-2013
Stem cells: What they are and what they do
Mayo Clinic Staff.  Retrieval date 2-19-2013
Pros and Cons of Stem Cell Research
Retrieval date 2-19-13
Human Biology. Sixth Edition. Michael D. Johnson

Part 2
1.       In this unit there were two activities I was proud of. The first assignment was the discussion on the autism/vaccination link. This activity had the potential to take me out of my comfort zone. I vaccinate both of my children and have felt firmly that I didn’t believe in a link between vaccines and autism. I worried that I would not be able to adequately express both sides of the debate since I felt so strong and comfortable in my stance. I feel like I did a good job with the research and the relaying of my thoughts and ideas. The second activity I am proud of was the molecules lab. That was a really challenging assignment for me! I like things to be black and white and crystal clear and that lab was not. After spending some time pouring over the book and doing some research on the internet and then the video you provided as assistance I felt a bit more comfortable and was able to complete the assignment pretty well.
2.       I have a really good understanding of the cell after this unit. I found it surprising how much I remembered from biology classes I had taken previously, some many years ago. I enjoyed learning about cell activities and how things inside of us work, so much complexity but I felt like I was able to get a good grasp on the information.
3.       To enhance my learning experience I tried to apply what we were learning to my life. In chapter three I read about cells that store fat. I learned that dieting and exercise tend to reduce the amount of stored fat, making the fat cells leaner but that dieting and exercise do not reduce their number. The cells are still available to store fat and that is why it is so hard to keep weight that you have lost off. I have dabbled in different diets and exercise plans to work on my health and this was just a relative connection to me and something that I really didn’t know.  I just knew it is very easy to pack the weight on but so hard to get it off!
4.       I felt most distanced while studying chapter two. I’m not sure what it was about atoms, elements, and the like but I spent a fair amount of time feeling confused.  I had to go over the information a few times to try to really take it in and I still feel like I struggled with some of the chemistry questions on exam 1. In unit one I felt most connected during labs and hands on activity, sometimes the reading was hard to get through but to see the information in action in front of me was really helpful.
5.       I honestly feel like the information in unit 1 was relayed well. There are so many options to reinforce the information and practice it between videos, labs, discussion, etc. I think the only suggestion I would have is something hands on or interactive with some of the chemistry involved in chapter 2 since that is where I feel like I struggled some.

Monday, February 18, 2013

Kitchen Fun!

Milk

*Observe and explain changes occurring in milk due to biological influence.

Place 1/2 cup of milk in a container and place in a warm, dark place for about 3 days.

Hypothesis- I believe the milk will sour, smell badly, and get chunky, due to the warmth and the growth of bacteria.

I placed my milk on the top shelf of our coat closet, it is dark in there and as warm as the rest of the house. The first couple of days I did not see any significant change in appearance or smell. Today was day three and there were some changes, there was a thickish ring around the edge of the cup and the milk seemed thicker in general and definitely did not look as white as the nice fresh milk I had put in three days prior, it had some yellowish tint to it.  I stirred it up and it was thick and smelled slightly sour. When I dumped the milk out there was a thick but smooth white substance on the bottom of the cup that reminded me of yogurt. When I tasted the milk it tasted just fine... just kidding! Ha!

I expected more changes but I think that the closet I put the milk in could have been warmer, there wasn't enough warmth for bacteria to grow more rapidly. Because milk contains bacteria and also lactose which bacteria happen to thrive on, the lactose sugar is changed to lactic acid which tastes sour and causes curdling.

Fresh milk on day one: Nice and white, bubbles, smelled like fresh milk


















Milk after three days: Yellowish, ring around cup, thick when swirled and stirred, slightly sour odor.




















Juice

*Witness mixing action of molecules and describe the process.

In all pictures far left is room temperature, middle is ice water, and right is boiling hot water. I used Welch's grape juice.

My hypothesis is that the water will mix well into the room temperature and boiling water but maybe not as well into the ice water. 

Preparation











 




Top view after grape juice added
















Front view after grape juice added















The grape juice immediately turned all three glasses of water shades of purple. In the first cup with the room temperature water it seemed to disperse well but after a few moments I could clearly see that it was clearer at the top of the glass which can be seen in the picture above. The middle glass is the ice water and again there is a clear separation at the top of the glass. The third cup was the boiling water and from my straight eye view it seemed to mix up the most evenly and was the most consistent in color. I think the colder water made the juice particles move more slowly which is why it took longer to disperse out and in the warm temperature and boiling water it was closer to the temperature of the room temp juice that I used and it was easier for the molecules to mix together. The ice water did seem to have the richest purple color below the separation.

Carrots

*Investigate cellular functions and cellular structures using a carrot.

My hypothesis for this experiment is that the carrot placed in the fresh water may expand some due to the water going into the carrot cells and causing them to burst. The carrot that is placed in salt water will have some shrinkage due to the salt water being hypertonic and drawing water out of the carrot cells.

Initial length and width of carrot pieces:
Piece 1- 4.125 inches long 7/8 inch wide
Piece 2- 4 1/4 inches long 7/8 inch wide

After-experiment length and width of carrot pieces
Piece 1- 4 1/2 inches long 7/8 inch wide
Piece 2- 4 inches long 3/4 inch wide

Carrots in water. Fresh water on left, salt water on right





Carrots prior to water baths.


Carrots after water bath

After 24 hours in the water, there were some changes. On the fresh water carrot the string was tighter then when I had first tied it the night before, the carrot was still very crisp and fresh feeling. The measurements grew a slight bit leading me to believe that some of the cells inside were burst because of the intake of water. Pure water is the most hypotonic solution so water would enter the cells, they would then swell and burst.

On the salt water carrot there was shrinkage. Because the salt water is hypertonic water diffused out of the carrot cells causing the shrinkage. The carrot was much softer then the fresh water carrot and the string had loosened up quite a bit.